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1-25 of 53 Business Insights

  • Application Note

    A Quantitative Determination of Methadone and its Metabolite (EDDP) in Dry Blood Spots Using LC-MSMS

    The present research study provides a much simplified approach whereby a highly sensitive LC-MS/MS triple quadrupole mass spectrometer is used to measure directly the methadone and EDDP concentrations through the dried blood spot (DBS) samples.

  • Application Note

    Rapid, High Throughput Analysis of Compounds in Urine

    Urine is the matrix of choice as it can be collected easily and in large volumes. The variations within the urine matrix can adversely impact chromatographic separation and LC-MS/MS signal. The present study demonstrates that a simple “dilute and shoot” method coupled with the high sensitivity QSight? 220 triple quadrupole mass spectrometer system eliminates many of the complexities of sample preparation without compromising quantitation quality.

  • Application Note

    High Sensitivity Methotrexate Quantitation in Human Serum Utilizing

    This application note outlines a rapid LC-MS/MS research method utilizing the QSight? 220 triple quadrupole mass spectrometer. The developed method provides exceptional results for the quantitation of methotrexate in serum especially in terms of LLOQs and linearity.

  • Application Brief

    HPLC Analysis of the Synthetic Antibiotic Ciprofloxacin Using Quasar Biphenyl Column

    Ciprofloxacin was the first fluoroquinolone brought to the market, in 1987, and represented an important medical breakthrough driving further research into this new class of antibiotics. It was developed in 1981 by Bayer and is currently the most potent of available fluoroquinolones. A broad-spectrum antibiotic it is used to treat several bacterial infections. Literature citations reference the use of a C18 HPLC column. However, due to the aromatic functionality with ciprofloxacin it makes it an ideal candidate for the application of a phenyl based stationary phase. This application brief describes use of a Quasar biphenyl column in the analysis of the synthetic antibiotic ciprofloxacin.

  • Application Note

    Determination of Coumarin in Electronic Cigarette Liquids by LC/MS/MS

    Coumarin (1,2-benzopyrone) occurs as a natural component in some plants, and is utilized as a flavoring ingredient in some foods, tobaccos and cosmetic products. In recent years, coumarin has been added to electronic cigarette liquid nicotine solutions as a flavoring component, producing a complex flavor profile with aromas of vanilla, almond, cherry and cinnamon. Coumarin has been found to exhibit hepatoxic effects, and has been banned by the FDA in food products, but this ban does not extend to tobacco products, such as electronic cigarettes.

    This application note details a method for the analysis of coumarin in electronic cigarette liquid nicotine cartridges, utilizing PerkinElmer UHPLC system coupled to a QSight? 220 triple quadrupole mass detector with a dual ionization source.

  • Application Note

    Analysis of 24 Polar and Non-Polar Volatile Organic Compound (VOCs) Emissions from Stationary Source

    Volatile organic compounds (VOCs) have been recognized as a primary source of air pollution, and are known photochemical reaction precursors which facilitate the production of tropospheric, or ground-level, ozone. Ground-level ozone can have significant impacts on human health, and contributes to climate change throughout the world. VOC emissions from stationary sources, such as ducts, stacks and exhaust funnels in factories, refineries and mines, have been identified as significant contributors to air pollution and ground-level ozone formation. In this paper, a qualitative and quantitative method for the analysis of 24 polar and nonpolar VOCs was established using a PerkinElmer TurboMatrix? ATD and a PerkinElmer Clarus? GC/MS with electron ionization (EI) source. The method is simple, sensitive and efficient to comply with regulatory requirements.

  • Application Brief

    HPLC Analysis of the Semi-Synthetic Antibiotic Azithromycin Using a Quasar C18 Column

    Azithromycin, discovered in 1980, is a semi-synthetic macrolide antibiotic derived from erythromycin. The drug is primarily used to treat various bacterial infections including respiratory infections, acute bacterial sinusitis, skin infections, Lyme disease, and some sexually transmitted infections. The mode of action of azithromycin is by inhibiting bacterial protein synthesis through binding to the 50S subunit of the bacterial ribosome, thereby inhibiting translation of mRNA. The USP monograph for the analysis of azithromycin states the use of a high pH mobile phase (pH 11), which requires a specific and expensive column. The method also uses amperometric electrochemical detection, which is often unavailable in many laboratories. HPLC with a UV or PDA detector provides a good alternative as it is available in most laboratories. This application brief describes the use of a Quasar C18 column in the analysis of azithromycin.

  • Application Brief

    HPLC Analysis of Ibuprofen Using Quasar C18 Columns in Accordance with the United States Pharmacopeia

    Ibuprofen belongs to the class of compounds known as Nonsteroidal Anti-inflammatory Drugs (NSAIDS) which are used in the treatment of pain, fever and various types of inflammation. NSAIDS work by inhibiting the synthesis of a wide range of prostaglandins produced as a response to injury, strain or illness. Since its introduction in 1969, its tolerability and effectiveness have made it one of the most popularly prescribed/taken medications in the world, with over 24 million prescriptions in the U.S. alone. Ibuprofen has generic status with many manufacturers, making it a very commercially important compound. Projections have estimated a global market value of ibuprofen to be 294.4 million USD in 2020, with an estimated value of 447.6 million USD by 2026. This application brief describes the use of a Quasar C18, on both porous silica and SPP silica phases for the analysis of ibuprofen in accordance with the official USP monograph, specifically, the chromatographic purity section.

  • Application Brief

    Robust Simvastatin Analysis Under High Aqueous Conditions

    Statins are a group of medicines that can help lower the level of low-density lipoprotein (LDL) cholesterol in the blood and help prevent cardiovascular disease. LDL cholesterol is often referred to as "bad cholesterol", and statins reduce its production in the liver by competitively inhibiting HMG CoA reductase, an enzyme involved in cholesterol synthesis. The origin of simvastatin can be found as far back as the 1950’s, when scientists at Merck were researching the biosynthesis of cholesterol. But it was the isolation of HMG CoA reductase by Japanese biochemist Akira Endo whose research into the relationship between fungi and cholesterol biosynthesis was the springboard for the development of the synthetic statin Simvastatin, marketed under the trade name of Zocor. This application brief describes the robust HPLC analysis of the polar compound Simvastatin using a gradient which reaches 100% aqueous conditions.

  • Application Note

    Speciation of Hexavalent Chromium in Children's Toys According to EN 71-3 Category II with the NexSAR HPLC-ICP-MS

    Testing for harmful metal(loid)s that may be included in raw materials, processes, paints and additives used in the manufacturing processes of children's toys has been mandated by the regulation EN 71-3. This regulation recommends specific testing methods and maximum allowable concentrations of leachable metals and metalloids such as Al, As, B, Ba, Cd, Co, Cr (III), Cr (VI), Cu, Hg, Mn, Ni, Pb, Sb, Se, Sn, organotin, Sr and Zn in parts of toys, with the exclusion of general packaging materials.

    This work shows a method for the measurement of hexavalent chromium in different toy material categories in accordance with method EN 71-3 Category II, leveraging the unique combination and capabilities of the NexSAR Inert HPLC coupled to the robust NexION® ICP-MS.

  • Application Brief

    Sulfa Drugs Analysis Using Quasar C18 HPLC Column

    Sulfa drugs are accredited as the first set of compounds to exhibit antibiotic properties used to prevent bacterial infections in humans. The first sulfonamide, trade named Prontosil, was developed by Bayer in 1932. The subsequent years saw rapid development of antibacterial drugs and by the 1940’s sulfanilamide was widely used. Though the medicine was relatively safe, allergic reactions such as skin rashes, fever and nausea were common place. With the introduction of less-toxic derivatives and especially with the mass production of penicillin, its use declined. These short acting synthetic sulfa drugs are effective against a wide range of pathogenic microorganisms and are now commonly used in veterinary medicine. Sulfathiazole administered to cattle in combination with penicillin and chlortectracyline has been shown to yield higher rates of weight gain and improved feed efficiency. This application brief illustrates the efficient separation of three sulfa drugs using the Quasar C18 HPLC column.

  • Application Note

    Chromium Speciation in Drinking Water by LC-ICP-MS

    Knowing the concentration of hexavalent chromium in environmental systems and samples which will be consumed is more important than knowing the total chromium concentration. This is especially true for drinking water. This work describes the use of LC-ICP-MS to measure both trivalent and hexavalent chromium in drinking waters, with the goal of presenting a suitable methodology for analyzing the range of concentrations encompassed by the new legislation.

  • Application Note

    Rapid Determination of Trace Amounts of Aniline and Its Derivatives by Direct Injection UHPLC/MS/MS

    Aniline and aniline derivatives are widely used as raw materials and intermediates in the polymer, rubber, dye, pesticide and pharmaceutical industries. Unintended releases of aniliane or its derivatives into the environment pose a serious threat, with release possible during any stage of production, storage, transport, use, or disposal. Therefore, it is important and necessary to develop fast, simple, sensitive, selective and efficient methods for the determination of aniline and its derivatives in drinking and environmental waters. The aim of this study is to develop an LC/MS/MS method for rapid analysis of aniline and its derivatives in water samples by direct injection, without using time consuming sample preparation steps.

  • Application Note

    UHPLC-PDA Analysis of Curcuma Using a Quasar SPP Column

    Turmeric is the powdered dry rhizome of the plant Curcuma longa L., and is used as a coloring agent to give a yellow-orange color to food dishes.

    It has been found to be a rich source of phenolic compounds, namely, three curcuminoids: curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Research to date suggests that turmeric, in addition to having an immunomodulatory role, is also of use in preventing oxidative stress that can lead to inflammation, cancer, and arthritis. This application note describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of the three curcuminoids in commercially available turmeric spices and roots by UHPLC, using photodiode array (PDA) detection.

  • Application Brief

    Arsenic Speciation Analysis in Rice Following Method GB 5009.11-2014 to Ensure Safety

    Rice represents a dietary staple for over half of the world's population. However, in recent years, concerns have been raised over the presence of high levels of arsenic (As), where this carcinogenic metalloid can be accumulated at levels far exceeding ambient concentrations. Since China is the world's largest producer of rice, the Chinese government released a national standard method in 2014 for food safety – GB 5009.11– to aid the evaluation of As species in rice and rice products. In this document, HPLC-ICP-MS is identified as the preferred analytical technique.

    In this study, five arsenic species were characterized in a commercial rice sample and certified reference material using a gradient anion-exchange method in accordance with GB 5009.11. The analysis was performed using a PerkinElmer NexSAR HPLC-ICP-MS speciation solution, consisting of a NexSAR inert HPLC coupled to a NexION® ICP-MS.

  • Application Brief

    Analysis of Water-Soluble Vitamins (B2, B3) using Quasar SPP HILIC Column

    In 1906, English biochemist Sir Frederick Gowland Hopkins also discovered that certain food factors were important to health. All the vitamins we recognise today were discovered during the early 20th century. There are two groups of vitamins, fat soluble and water soluble. Both types are regarded as essential for normal growth and our overall well being. Vitamin B2 (Riboflavin) and vitamin B3 (Niacinamide) are water soluble vitamins, naturally occurring in a number of food stuffs including milk and eggs. Both vitamins are used as dietary supplements and can help lower cholesterol, prevent migraines. Hydrophilic interaction liquid chromatography (HILIC), as first suggested by Alpert in 1990 provides an alternative approach to effectively separate small polar compounds, on polar stationary phases, which are not well retained under reversed phase conditions. Water is the strongest solvent in HILIC and as mobile phases typically contain low levels of water the resulting methods are ideally suited for MS applications. This application brief illustrates the efficient separation of vitamin B2 and B3 applying the HILIC mode of separation using the Quasar SPP HILIC column.

  • Flyer

    NexSAR HPLC-ICP-MS Speciation Solution

    Speciation studies can provide comprehensive information regarding ion mobility, bioavailability, and toxicity. Consequently, many modern labs are expanding their analytical portfolio to include HPLC-ICP-MS. For such studies, it is advantageous to have an intuitive, user-friendly system that meets the user requirements for automation, ultra-low baseline, powerful integration, and flexible data export. And no one offers a more integrated, versatile, and inert speciation solution than PerkinElmer - accelerate your speciation analysis with the NexSAR? HPLC-ICP-MS Speciation Solution.

  • Application Brief

    HPLC Analysis of Omeprazole Using a Quasar C8 Column in Accordance with the United States Pharmacopeia

    Omeprazole belongs to a class of drugs known as Proton Pump Inhibitors (PPI’s). It is used in the treatment of conditions relating to overactive stomach acid such as gastroesophageal reflux disease, peptic ulcers and Zollinger-Ellison syndrome. The World Health Organization regards omeprazole among its ‘List of Essential Medicines’ which outlines the most effective and safest medicines required by a countries healthcare system to meet its needs. Omeprazole is a very commercially important drug, being ranked as the 7th most prescribed drug in the USA in 2017 with over 58 million prescriptions. This application brief describes the use of a Quasar C8 column for the analysis of omeprazole in accordance with the United States Pharmacopoeia (USP) monograph.

  • White Paper

    Advantages and Applications of Superficially Porous Particle Columns in Liquid Chromatography

    The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superficially porous particles has received considerable attention. This white paper gives an overview about the theory behind the success of superficially porous particles technology and presents a summary of its latest applications.

  • Application Brief

    Robust Atorvastatin Analysis Under High Aqueous LC Conditions Application Brief

    The origin of statins dates back to the mid 1970’s when the Japanese biochemist, Akira Endo, isolated a factor from the fungus Penicillium citrinum which he identified as a competitive inhibitor of 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMG-CoA reductase). This substance, which he named compactin or mevastatin, was the first statin to be administered to humans. Statins competitively inhibit HMG-CoA reductase, an enzyme involved in cholesterol synthesis, especially in the liver. Used to prevent cardiovascular disease and to lower cholesterol levels, atorvastatin is one of a number of synthetic statins routinely produced. It is now the most commonly prescribed statin medication. This application brief describes the robust HPLC analysis of the polar compound atorvastatin using a gradient which reaches 100% aqueous mobile phase conditions.

  • Application Note

    Cannabinoid Monitoring in Dried Cannabis Flowers

    This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

  • Application Note

    Determination of 24 Polycyclic Aromatic Hydrocarbons in Smoked Meat by ASE Extraction-GPC Purification Coupled with GC/MS

    Polycyclic aromatic hydrocarbons (PAHs), a class of complex compounds containing two or more benzene rings, are widely found in the environment and food. PAHs are formed during the incomplete combustion of organic matter, such as wood utilized in cooking and food preparation. They have attracted wide attention due to studies which have shown the teratogenicity and carcinogenicity of PAH compounds. For humans, food intake is one of the main sources of PAH exposure, and can be the result of contamination from anthropogenic sources, food processing or cooking practices.

    In this paper, a validated method employing accelerated solvent extraction (ASE) and gel permeation chromatography (GPC), followed by solid phase extraction (SPE) on silica and analytical determination by GC/MS was applied for the detection of PAHs in smoked meat. The results demonstrate that the method is suitable for the simultaneous determination of 24 PAHs in smoked meat with good efficiency, accuracy and reproducibility.

  • Application Note

    Cannabinoid Monitoring in a Variety of Edibles

    This application describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of twelve primary cannabinoids in the extracts of several food matrices by HPLC, using photodiode array (PDA) detection. The method provides exceptional chromatographic repeatability and affords LOQs well below the current concentration levels of interest for cannabinoids in edibles. Thereupon, the method/procedure defined herein can be expected to fulfill the essential task of ensuring product uniformity and cannabinoid screening in edible foods.

  • Application Brief

    HPLC Analysis of Tramadol Using SPP Column Technology

    Opiates, originally derived from the opium poppy, have been used for thousands of years for both recreational and medicinal purposes.

  • Application Brief

    HPLC Analysis of Fluconazole Using Quasar C18 Columns in Accordance with USP Application Brief

    Fluconazole is an azole anti-fungal medication used primarily in the treatment of a wide range of fungal infections. Examples include thrush, urinary tract infections and the prevention of fungal infections in immunocompromised patients undergoing chemotherapy. The mechanism of action can be described as fungistatic, the growth and replication of fungi is inhibited by fluconazole rather than directly attacked. This process is achieved by inhibiting enzymes which are important in the production of fungal membranes, allowing the immune system ample opportunity to target and degrade the pathogen. Fluconazole has generic status and is thus globally produced and prescribed. It is on the World Health Organization’s (WHO) list of essential medicines, which serves as a model of the safest and most effective medications needed by a health system. This application brief describes the use of a Quasar C18, on both porous silica and SPP silica phases, for the analysis of fluconazole in accordance with the official USP monograph.

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