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  • Application Note

    Screening for Inhibitors of PD-1 and PD-L1 Binding with AlphaLISA Technology

    Many tumor cells, which under normal, healthy conditions would be recognized by the body’s T cells and thereby targeted for destruction, have developed ways to evade the host immune system by taking advantage of immune checkpoint pathways. Among the most promising approaches to activating therapeutic antitumor immunity is through the blockade of immune checkpoints. The programmed cell death-1 (PD-1) immune checkpoint pathway is a negative regulator of T-cell immune function. When PD-1 is bound to programmed cell death-ligand 1 (PD-L1), T cell response is suppressed. Inhibitors that block PD-1/PD-L1 complex formation lead to increased activation of T-cells and immune system functions, allowing the body’s immune system to identify and attack tumor cells. So far, several anti-PD-1 or PD-L1 monoclonal antibodies have been developed to treat a variety of cancers, including non-small cell lung carcinoma (NSCLC), metastatic melanoma and renal cancer. The promise of therapeutically exploiting this pathway has created a need for more robust, straight-forward assays to identify and qualify potential inhibitors which interrupt PD-1/PD-L1 binding. Find out how AlphaLISA? technology provides a simple, homogenous, straightforward method for detecting PD-1/PD-L1 binding.

  • Application Note

    Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

    Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

    Alpha technology offers a simple, straight forward workflow. No wash needed!

    1. Add sample
    2. Add Acceptor bead mix; incubate 1 hr
    3. Add Donor beads; incubate 30 mins
    4. Read on Alpha-enable microplate reader

    Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

  • Application Note

    Alpha Technology: A Fast and Sensitive Orthogonal Approach to Cell-based Potency Assays

    Potency estimates play a central role in drug development. It is imperative that orthogonal platforms to cell-based assays be established for cross validation as a complementary strategy directive per regulatory guidelines.

    Download this application note to see how AlphaLISA? bead-based, homogeneous assays proves an ideal orthogonal approach to cell-based potency assays for a biotherapeutics system

  • Application Note

    Measuring PD-L1 Expression in Breast Cancer Cell Lines with AlphaLISA

    Too many candidates, too little time. The lack of robust, rapid, high-throughput assays to identify and qualify potential therapeutic targets in areas such as cancer research continues to cost valuable time. What if you could increase assay throughput without compromising sensitivity, obtain more data points from each sample and eliminate tedious wash steps? Find out how AlphaLISA? assay technology, combined with the EnVision? multimode plate reader, provides a fast, powerful, homogeneous platform for screening potential inhibitors of PD-L1 (a protein associated with breast cancer tumor cells) expression in human cells.

  • Application Note

    Evaluating the Specificity of PD-1 and PD-L1 Blocking Antibodies Using AlphaLISA Human and Mouse PD-1/PD-L1 Binding Kits

    Mouse pharmacological models continue to play a large role in the study of human disease, and mouse tool reagents have shown high utility in immunology and cancer research for decades. It can often be quicker to learn about immunology and the regulation of immune responses using a syngeneic mouse model. However, working in mouse systems can often require the development of separate mouse reagents, if the therapeutic agent of interest does not cross-react with mouse. Find out how the AlphaLISA? human PD-1/PD-L1 and AlphaLISA mouse PD-1/PD-L1 binding assays provide a fast, powerful, homogeneous platform for obtain binding potencies from potential novel drug candidates.

  • Application Note

    Applicability of DELFIA Fluorescence-Based Immunoassay Technology to Detect and Quantitate Biomarker

    DELFIA® (Dissociated-Enhanced Lanthanide Fluorescent Immunoassay) TRF is a proven, robust immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal, and improved assay dynamic range.

    Download this application note to see how DELFIA time-resolved fluorescence (TRF) technology serves as an alternative to traditional ELISA with a wider dynamic range and superior signal stability for quantitation of biomarkers in complex sample types, including serum, plasma, and blood.

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